Insoluble Proteins: Methods and Protocols cover image

Insoluble Proteins: Methods and Protocols

By Elena García-Fruitós (eds.)
pdf
Format
2015
Year
English
Language
Humana Press
Publisher

Summary

<p><p>With insolubility proving to be one of the most crippling bottlenecks in the protein production and purification process, this volume serves to aid researchers working in the recombinant protein production field by describing a wide number of protocols and examples. <i>Insoluble Proteins: Methods and Protocols</i> includes chapters that describe not only the recombinant protein production in different expression systems but also different purification and characterization methods to finally obtain these difficult-to-obtain proteins. Beginning with protein production methods using both prokaryotic and eukaryotic expression systems, the book continues with purification protocols using insoluble proteins, the characterization of insoluble proteins, as well as a general overview of interesting applications of insoluble proteins. Written in the highly successful <i>Methods in Molecular Biology</i> series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.</p><p>Comprehensive and practical, <i>Insoluble Proteins: Methods and Protocols</i> aims to provide the scientific community with detailed and reliable state-of-the-art protocols that are used in order to successfully produce and purify recombinant proteins prone to aggregate.</p></p>

Related books


Others
CRC Handbook of Chemistry and Physics 90th ed [internet ed]
D. Lide
Mathematics
Il Calcolo Differenziale ed Integrale reso Facile ed Attraente
Gustavo Bessiere
Others
Erectile Dysfunction Cure: How To Cure ED Naturally & Quickly & Enjoy Your Intimate Life (Jelqing, Male Enhancement, ED Cure, Erectile Dysfunction, Infertility)
Gregory Moto
Others
Erectile Dysfunction Cure: How To Cure ED Naturally & Quickly & Enjoy Your Intimate Life (Jelqing, Male Enhancement, ED Cure, Erectile Dysfunction, Infertility)
Gregory Moto
Others
Aadam ed Eeva
Arto Passilinna
Others
ANABOLICS, 10th ed.
William Llewellyn